Device

Part:BBa_K5477046:Design

Designed by: Kate Malana Escobar   Group: iGEM24_UCopenhagen   (2024-09-28)


SUPERMOM: Dual-Function Biosensor for BPA Detection and Detoxification


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6656
    Illegal XbaI site found at 1606
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
    Illegal PstI site found at 3825
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6656
    Illegal NheI site found at 182
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
    Illegal PstI site found at 3825
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6656
    Illegal BglII site found at 1740
    Illegal BglII site found at 2751
    Illegal BglII site found at 4405
    Illegal BamHI site found at 6476
    Illegal BamHI site found at 6770
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6656
    Illegal XbaI site found at 1606
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
    Illegal PstI site found at 3825
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6656
    Illegal XbaI site found at 1606
    Illegal PstI site found at 1790
    Illegal PstI site found at 1961
    Illegal PstI site found at 3825
    Illegal AgeI site found at 37
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In this biosensor system, the combination of a mutant ERα receptor module, a reporter module, and a detoxification system provides a robust setup for detecting and detoxifying estrogen-like compounds, particularly bisphenol A (BPA). The integration of these modules allows for ligand detection, signal transduction, and metabolic detoxification all within a single yeast-based system.



Source

Homo sapiens, S. cerevisiae and E. coli


References